ISOLATION AND SOME CHARACTERISTICS OF PLACENTAL ALKALINE PHOSPHATASE
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چکیده
منابع مشابه
Nucleotide and amino acid sequences of human intestinal alkaline phosphatase: close homology to placental alkaline phosphatase.
A cDNA clone for human adult intestinal alkaline phosphatase (ALP) [orthophosphoric-monoester phosphohydrolase (alkaline optimum); EC 3.1.3.1] was isolated from a lambda gt11 expression library. The cDNA insert of this clone is 2513 base pairs in length and contains an open reading frame that encodes a 528-amino acid polypeptide. This deduced polypeptide contains the first 40 amino acids of hum...
متن کاملPlacental alkaline phosphatase as the placental IgG receptor.
We review data from our studies of the physiological role of placental alkaline phosphatase (PLAP) and report that, in addition to functioning in catalysis, PLAP has the capacity to bind the Fc portion of human IgG. The dissociation constant for the interaction (3.86 mumol/L) indicates that the PLAP-IgG complex probably occurs in vivo. Furthermore, the electrophoretic and immunochemical propert...
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An alkaline phosphatase (EC 3.1.3.1) of the placental type was isolated from a seminoma type of human testicular cancer tissue and was purified to homogeneity by sulfate-mediated chromatography on a column of Cibacron Blue Sepharose 4B. The purified enzyme had a specific activity of 40.6 kU per gram of protein and was obtained in a yield of 37%. The purification procedure used was simple and ec...
متن کاملIsolation and molecular identification of bacteria producing alkaline phosphatase enzyme from environmental sources
Alkaline phosphatase (ortho phosphate, monoester hydrolase phosphoinositide E. C. 3.1.3.1) is a non-specific metalloproteinase enzyme that is located inside periplasmic space of bacteria. This enzyme is used to measure freshwater sediment in genetic engineering for cleaning water. Isolation and molecular identification of the bacteria producing alkaline phosphatase and comparison of its product...
متن کاملCharacterization of KB cell alkaline phosphatase. Evidence of similarity to placental alkaline phosphatase.
The alkaline phosphatase from KB cells was purified, characterized, and compared to placental alkaline phosphatase, which it resembles immunologically. Two nonidentical nonomeric subunits of the KB phosphatase were found. The two subunits, which have apparent molecular weights of 64,000 and 72,000, can be separated on polyacrylamide gels containing sodium dodecyl sulfate. The Mr = 64,000 KB sub...
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ژورنال
عنوان ژورنال: The Kurume Medical Journal
سال: 1977
ISSN: 0023-5679,1881-2090
DOI: 10.2739/kurumemedj.24.7